Ion AmpliSeq™ Library Prep

Prepare NGS samples for amplicon-based targeted resequencing

The Ion AmpliSeq™ Library Prep application manages sample data for the Ion AmpliSeq Library and Ion AmpliSeq HD Library Preparation workflows. Process extracted DNA, RNA or FFPE samples through library prep and pooling with the Ion Torrent™ Ion Chef™ System or manual preparation. Metadata generated from the creation of Ion libraries is captured centrally in the app, including reagent kit, lots consumed, as well as equipment and instruments utilized (such as thermocyclers and pipettes) to support downstream template preparation and chip loading. Scientists can use this app to establish a full chain of custody for all sample prep activities and to provide a searchable archive for tracking the reliability and quality of processes.

Functionality

  • Supports automated (Ion Chef System or liquid handlers) and manual library preparation
  • Provides traceability of sample and container lineage including concentration and volume
  • Assign library barcodes for identification during pooling, sequencing, and downstream analysis
  • Track prepared amplicon library data for downstream sequencing on Ion PGM™, Ion Proton™, Ion S5™, and Ion GeneStudio S5 sequencing instruments
  • Generate sample set file for import to the Torrent Suite™ Software
  • Manually assign Ion barcodes to libraries or scan IonCode™ PCR plates for automated assignment
  • Maintain a full chain of custody for all sample preparation data
  • Easily troubleshoot processes by querying data collected by the app
  • Use in conjunction with the NGS Lab QC app to assess the quality of your Ion AmpliSeq libraries as well as with the Illumina Flow Cell Prep and Sequencing app
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