PacBio SMRT Sequencing

Design sequencing run and generate sample sheet

The PacBio SMRT Sequencing application enables scientists to generate SMRTbell templates from their prepared libraries. The process tracks the reagents for annealing, binding and clean-up as well as size selection. Prepared plates are then queued up for sequencing on the RSII or Sequel instruments. The sequencing process generates a samples sheet for the instrument including the sample ids, well location, library adapters, reagent kit lots and additional run parameters. Administrators can configure functionality from Thermo Scientific™ Core Connect™ software to post the run design to the Sequel instrument.

Functionality

• Design, run, and generate sample sheet
• Track the reagents, plate barcode, sample ids and library adapters of sequencing run
• Leverage Core Connect™ software to post run design to the Sequel instrument
• Optionally pool prepped libraries in preparation for template preparation and sequencing
• Use in conjunction with PacBio RT PCR and PacBio SMRT cDNA Library Prep applications