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The PacBio SMRT Sequencing application enables scientists to generate SMRTbell templates from their prepared libraries. The process tracks the reagents for annealing, binding and clean-up as well as size selection. Prepared plates are then queued up for sequencing on the RSII or Sequel instruments. The sequencing process generates a samples sheet for the instrument including the sample ids, well location, library adapters, reagent kit lots and additional run parameters. Administrators can configure functionality from Thermo Scientific™ Core Connect™ software to post the run design to the Sequel instrument.